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Ludovic Renault, PhD
Email: llrrenault@gmail.com
• Single particle analysis of DNA binding proteins. One publication associated so far with these projects.
• Single particle and electron crystallography analyses of membrane proteins. Dr. Stahlberg’s laboratory is part of the Center for Structures of Membrane Proteins (http://csmp.ucsf.edu/index.htm). This resulted so far in one publication.
• I participated in the development of an algorithm to automatically detect crystal lattice for two-dimensional crystals. This project has led to one publication.
• During this period I also wrote three reviews. One on single particle techniques and two on electron crystallography of membrane proteins, and I taught sample preparation for electron microscopy during the EMS/MCB 230 master’s course.
• I am the lab manager, responsible for maintaining stocks and ordering products. I also manage undergraduate students.
• Set up and fulfilled structure function relationship projects involving synaptic proteins and/or their structural homologues. These projects resulted in two publications.
• Solved two crystal structures in collaboration with Dr. Yves Bourne (AFMB, Marseille, France). These structures not yet published could result in two publications.
• Contributed to the development of the ESTHER database (http://bioweb.ensam.inra.fr/ESTHER/general?what=index). This contribution resulted in two publications and one oral presentation during an international conference.
• Supervised trainees at the undergraduate level.
• Purified, analyzed physico-chemically and functionally, and crystallized two macromolecules implicated in the architecture or the functioning of cholinergic synapses.
• The results of this six months research allowed me to write a research project for the PhD and obtain fellowships from “Association France Alzheimer” and “Association Française contre les Myopathies”.
Subject: Structural approach of the acetylcholinesterase inhibition by monoclonal antibodies.
Fellowships: “Association France-Alzheimer” (2003) and “Association Française contre les Myopathies” (2004 and 2005).
Report for the research internship: Renault L. (2002) Biochemical and structural studies of macromolecules implicated in the architecture and the functioning of the cholinergic synapses [in French].
Bibliographic for the 2nd year: Vallet M.C. & Renault L. (2001) Multistationarity as a possible model for epigenesis [in English].
• Mass-spectrometry, electrophoreses and western blot using the Phast System, UV quantification, colorimetric assays, Diffusion Light Scattering.
• Enzyme activity and inhibition assays.
• Crystallogenesis, collection and processing of x-ray data, structure determination by molecular replacement, refinement.
• Laureate for the « CCP4 Study Weekend » of a fellowship (travel + registration fees + part of the price of the hotel), University of Reading, 6-8 January 2005.
• X-ray crystallography: AMoRe, Arp-Warp, CCP4, Coot, Denzo, MosFLM, Phaser, Refmac, Scala, Turbo-Frodo
• 3d homology modeling: Chemera/Bigger, Coot, Hex, Swiss-model webserver
French
Native language
English
Reading: fluent
Written and oral expression: professional (oral and written communications). Currently living and working in the USA since January 2006.
Liu J., Renault L., Stahlberg H., and Heyer W. (2009) Rad51-dsDNA filaments and their interactions with Srs2 and Rad55/57 proteins. To be published
Klammt C., Perrin M., Maslennikov I., Renault L., Krupa M., Kwiatkowski W., Stahlberg S., Vale W. and Choe S. (2009) Polymer-based cell-free expression and analysis of G-protein coupled receptors in the absence of detergents. To be published
Evans J.E., Renault L., & Stahlberg H. (2008) Structure determination using two-dimensional crystallography. In: 3D-Electron Microscopy in Life Sciences (a Textbook on DVD). Ed. A. Verkleij, E.V. Orlova, and A. Leis. First edition. 3DEM NETWORK of EXCELLENCE (European Union).
Zeng X., Gipson B., Zheng Z.Y., Renault L., and Stahlberg H. (2007) Automatic lattice determination for two-dimensional crystal images. J. Struct. Biol. 160(3), 353-361.
Renault L. and Stahlberg H. (2007) Three-dimensional electron microscopy of macromolecular assemblies (J. Frank), Book Review. Scanning. 29, 37.
Renault L., Chou H.T., Chiu P.L., Hill R.M., Zeng X., Gipson B., Zhang Z.Y., Cheng A., Unger V., and Stahlberg H. (2006) Milestones in electron crystallography. J. Comp.-Aided Molec. Design. 20, 519-527.
Renault L., Nègre V., Hotelier T., Cousin X., Marchot P., & Chatonnet A. (2005) New friendly tools for users of ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Chem. Biol. Interact. 157-158, 339-343.
Bourne Y., Hasper A.A., Chahinian H., Renault L., Juin M.A., de Graaff L.H., & Marchot P. (2005) A. niger protein « EstA », perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily. Chem. Biol. Interact. 157-158, 395-396.
Renault L., Essono S., Boquet D., Juin M., Grassi J., Bourne Y., & Marchot P. (2005) Structural insights into AChE inhibition by monoclonal antibodies. Chem. Biol. Interact. 157-158, 397-400.
Hotelier T., Renault L., Negre V., Marchot P., & Chatonnet A. (2004) ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Nucleic Acids Research 32, D 145-147.
Renault L., Chou HT., Chiu PL., Zeng X., Gipson B., and Stahlberg H. (2007) Structural Studies of Membrane Proteins. Electron Crystallography at UC Davis. Poster to the Workshop on Advanced Topics in EM Structure Determination. Scripps San Diego, USA.
Renault L, Gruswitz F., Stroud RM, and Stahlberg H. (2006) Regulation of the Ammonium Transporter: Electron Crystallography Study of the AmtB-GlnK Complex. Poster to the 2006 International Conference on Structural Genomics, Beijing, China. Young Scientist ISGO Travel Award.
Renault L, Essono S., Boquet D., Juin M., Grassi J., Bourne Y., & Marchot P. (2005) Structural and theoretical insights into inhibition of acetylcholinesterase by monoclonal antibodies. Poster to the International Congress of Myology, Nantes. AFM Fellowship.
Renault L, Negre V., Hotelier T., Marchot P., & Chatonnet A. (2004) ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Oral and poster communication during the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
Renault L, Essono S., Boquet D., Juin M., Grassi J., Bourne Y ., & Marchot P. (2004) Structural insights into AChE inhibition by monoclonal antibodies. Poster to the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
Bourne Y., Hasper A.A., Chahinian H., Renault L, Juin M.A., de Graaff L.H., & Marchot P. (2004) A. niger protein « EstA », perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily. Poster to the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
Biochemistry/Structural biology - research scientist, specialized on membrane proteins
EXPERIENCE / MAIN ACHIEVEMENTS
Since January 06, Post-Doctoral position in Cryo Electron Microscopy
Stahlberg Laboratory (UC Davis, CA, USA), http://stahlberglab.org
• Single particle analysis of DNA binding proteins. One publication associated so far with these projects.
• Single particle and electron crystallography analyses of membrane proteins. Dr. Stahlberg’s laboratory is part of the Center for Structures of Membrane Proteins (http://csmp.ucsf.edu/index.htm). This resulted so far in one publication.
• I participated in the development of an algorithm to automatically detect crystal lattice for two-dimensional crystals. This project has led to one publication.
• During this period I also wrote three reviews. One on single particle techniques and two on electron crystallography of membrane proteins, and I taught sample preparation for electron microscopy during the EMS/MCB 230 master’s course.
• I am the lab manager, responsible for maintaining stocks and ordering products. I also manage undergraduate students.
September 02 to December 05, Ph.D. training (advisor, Dr Pascale Marchot)
”Ingénierie des Protéines” laboratory (Marseille, France), http://crn2m.univ-mrs.fr/-ToxCIM-?lang=en• Set up and fulfilled structure function relationship projects involving synaptic proteins and/or their structural homologues. These projects resulted in two publications.
• Solved two crystal structures in collaboration with Dr. Yves Bourne (AFMB, Marseille, France). These structures not yet published could result in two publications.
• Contributed to the development of the ESTHER database (http://bioweb.ensam.inra.fr/ESTHER/general?what=index). This contribution resulted in two publications and one oral presentation during an international conference.
• Supervised trainees at the undergraduate level.
January to June 02, Six months Master’s research internship (advisor, Dr Pascale Marchot)
“Ingénierie des Protéines” laboratory (Marseille, France), http://crn2m.univ-mrs.fr/-ToxCIM-?lang=en• Purified, analyzed physico-chemically and functionally, and crystallized two macromolecules implicated in the architecture or the functioning of cholinergic synapses.
• The results of this six months research allowed me to write a research project for the PhD and obtain fellowships from “Association France Alzheimer” and “Association Française contre les Myopathies”.
EDUCATION
September 02 to December 05, Université de la Méditerrané (Marseille, France)
Ph.D. in Neurosciences, advisor Dr Pascale Marchot.Subject: Structural approach of the acetylcholinesterase inhibition by monoclonal antibodies.
Fellowships: “Association France-Alzheimer” (2003) and “Association Française contre les Myopathies” (2004 and 2005).
September 01 to June 02, Université de la Méditerranée (Marseille, France)
D.E.A. in Bioinformatics, Structural Biology and Genomic, equivalent to Master’s degree (four months courses + six months internship in a research laboratory)Report for the research internship: Renault L. (2002) Biochemical and structural studies of macromolecules implicated in the architecture and the functioning of the cholinergic synapses [in French].
September 99 to Sept 02, Université de Rouen (Rouen, France)
European Magister in Integrative Biology, equivalent to Master’s degree (3 years validated by the D.E.A)Bibliographic for the 2nd year: Vallet M.C. & Renault L. (2001) Multistationarity as a possible model for epigenesis [in English].
TECHNICAL SKILLS
Biochemistry
• Gel-filtration, ion-exchange, and immuno-affinity chromatographies by FPLC.• Mass-spectrometry, electrophoreses and western blot using the Phast System, UV quantification, colorimetric assays, Diffusion Light Scattering.
• Enzyme activity and inhibition assays.
Protein crystallization/crystallography
• Two-dimensional crystallization of membrane proteins, screening on the microscope, determination of 2d projection maps.• Crystallogenesis, collection and processing of x-ray data, structure determination by molecular replacement, refinement.
• Laureate for the « CCP4 Study Weekend » of a fellowship (travel + registration fees + part of the price of the hotel), University of Reading, 6-8 January 2005.
COMPUTER SKILLS
Structure programs
• Cryo-EM: 2dx, Eman, Spider• X-ray crystallography: AMoRe, Arp-Warp, CCP4, Coot, Denzo, MosFLM, Phaser, Refmac, Scala, Turbo-Frodo
• 3d homology modeling: Chemera/Bigger, Coot, Hex, Swiss-model webserver
Sequence analysis tools
• Similarity searches, primary structure analysis, secondary and tertiary structure prediction, alignmentsOther scientific softwares
• Prism GraphPad, Swiss-pdb Viewer, GRASP, PyMOL, VMD, Chimera, etcEnvironments
• MacOS, Windows and LinuxStandard softwares
• MS Word, MS PowerPoint, MS Excel, Adobe Photoshop, EndNote, etcLANGUAGES
French
Native language
English
Reading: fluent
Written and oral expression: professional (oral and written communications). Currently living and working in the USA since January 2006.
PUBLICATIONS
Liu J., Renault L., Stahlberg H., and Heyer W. (2009) Rad51-dsDNA filaments and their interactions with Srs2 and Rad55/57 proteins. To be published
Klammt C., Perrin M., Maslennikov I., Renault L., Krupa M., Kwiatkowski W., Stahlberg S., Vale W. and Choe S. (2009) Polymer-based cell-free expression and analysis of G-protein coupled receptors in the absence of detergents. To be published
Evans J.E., Renault L., & Stahlberg H. (2008) Structure determination using two-dimensional crystallography. In: 3D-Electron Microscopy in Life Sciences (a Textbook on DVD). Ed. A. Verkleij, E.V. Orlova, and A. Leis. First edition. 3DEM NETWORK of EXCELLENCE (European Union).
Zeng X., Gipson B., Zheng Z.Y., Renault L., and Stahlberg H. (2007) Automatic lattice determination for two-dimensional crystal images. J. Struct. Biol. 160(3), 353-361.
Renault L. and Stahlberg H. (2007) Three-dimensional electron microscopy of macromolecular assemblies (J. Frank), Book Review. Scanning. 29, 37.
Renault L., Chou H.T., Chiu P.L., Hill R.M., Zeng X., Gipson B., Zhang Z.Y., Cheng A., Unger V., and Stahlberg H. (2006) Milestones in electron crystallography. J. Comp.-Aided Molec. Design. 20, 519-527.
Renault L., Nègre V., Hotelier T., Cousin X., Marchot P., & Chatonnet A. (2005) New friendly tools for users of ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Chem. Biol. Interact. 157-158, 339-343.
Bourne Y., Hasper A.A., Chahinian H., Renault L., Juin M.A., de Graaff L.H., & Marchot P. (2005) A. niger protein « EstA », perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily. Chem. Biol. Interact. 157-158, 395-396.
Renault L., Essono S., Boquet D., Juin M., Grassi J., Bourne Y., & Marchot P. (2005) Structural insights into AChE inhibition by monoclonal antibodies. Chem. Biol. Interact. 157-158, 397-400.
Hotelier T., Renault L., Negre V., Marchot P., & Chatonnet A. (2004) ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Nucleic Acids Research 32, D 145-147.
COMMUNICATIONS
Renault L., Chou HT., Chiu PL., Zeng X., Gipson B., and Stahlberg H. (2007) Structural Studies of Membrane Proteins. Electron Crystallography at UC Davis. Poster to the Workshop on Advanced Topics in EM Structure Determination. Scripps San Diego, USA.
Renault L, Gruswitz F., Stroud RM, and Stahlberg H. (2006) Regulation of the Ammonium Transporter: Electron Crystallography Study of the AmtB-GlnK Complex. Poster to the 2006 International Conference on Structural Genomics, Beijing, China. Young Scientist ISGO Travel Award.
Renault L, Essono S., Boquet D., Juin M., Grassi J., Bourne Y., & Marchot P. (2005) Structural and theoretical insights into inhibition of acetylcholinesterase by monoclonal antibodies. Poster to the International Congress of Myology, Nantes. AFM Fellowship.
Renault L, Negre V., Hotelier T., Marchot P., & Chatonnet A. (2004) ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins. Oral and poster communication during the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
Renault L, Essono S., Boquet D., Juin M., Grassi J., Bourne Y ., & Marchot P. (2004) Structural insights into AChE inhibition by monoclonal antibodies. Poster to the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
Bourne Y., Hasper A.A., Chahinian H., Renault L, Juin M.A., de Graaff L.H., & Marchot P. (2004) A. niger protein « EstA », perhaps a new electrotactin, defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily. Poster to the VIIIth International Meeting on Cholinesterases, Perugia, Italy.
